Generator

Part:BBa_K215200:Design

Designed by: Alex Leone   Group: iGEM09_Washington   (2009-09-30)

Generic Surface Display Construct: IPTG Inducible Lpp + 1tmr OmpA + NheI Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We took the Lpp Signaling Peptide and OmpA 1 trans-membrane region from Havard 2006, BBa_J36848, with two custom primers:

  • Lpp Forward: 5'-gcggccgctTCTAGAtgaaagctactaaactggtactggg-3'
  • OmpA Reverse with GS Linker, GGGSGGGSGGG, and a TEV site, ENLYFQG, and SpeI site: 5'-cggccgctACTAGTaGCTAGCaccctgaaaatacaggttttcACCACCACCAGAACCACCACCAGAACCACCACCgctgcctttgtacggcatacgacc-3'

Source

The Lpp + 1tmr OmpA is from Havard iGEM 2006: BBa_J36848, which is from [cite].

References